The Sekelsky Lab

pP{whiteOut2}

pP{whiteOut2} is a P element transformation vector for ends-out (gene replacement) targeting in which the transformation marker (hsp70::white) is located outside of the FRTs. This allows targeting of a genomic fragment without the transformation marker. Of course, you'll need some way to screen for targeted events, such as a mutant phenotype or a different marker (e.g., GFP expression for targeting of a fusion construct).

pP{whiteOut2} Sequence

Updated 06/16/2008 (thanks for Nick Lowe for providing sequence correction near BamHI site).

CATGATGAAATAACATAAGGTGGTCCCGTCGGCAAGAGACATCCACTTAACGTATGCTT GCAATAAGTGCGAGTGAAAGGAATAGTATTCTGAGTGTCGTATTGAGTCTGAGTGAGAC AGCGATATGATTGTTGATTAACCCTTAGCATGTCCGTGGGGTTTGAATTAACTCATAAT ATTAATTAGACGAAATTATTTTTAAAGTTTTATTTTTAATAATTTGCGAGTACGCAAAG ctagagttgttggttggcacaccacaaatatactgttgccgagcacaattccgctcgac GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCTAGGGATAACAGGGTAATGGTACCC GCCCGGGGATCAGATCCGCGGCCGCACGCGTGAATTCGCTAGCATGCAGGCCTGCAGAT CTCGAGTAGGGATAACAGGGTAATGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCgga tccagatccccccgttattctctattcgttttgtgactctccctctctgtactattgct ctctcactctgtcgcacagtaaacggcacgctattctcgttgcttcgagagagcgcgcc tcgaatgttcgcgaaaagagcgccggagtataaatagaggcgcttcgtcgacggagcgt caattcaattcaaacaagCAAAGTGAACACATCGCGAAGCGTAAGCTGAGCAAACAAAC AAGCGCAGCTGAACAAGCTAAACAATCTGCAATAAAGTGCAAGTTAAAGTGAATCAATT AAAAGTAACCAACAACCAAGTAATTAAACTAAAAACTGCAACTACTGAAATCAACCAAG AAGTCATTATTGAAGACAAGAAGAGAACTCTgaataggtcgatagcgtcaattcggcac gaggcgtttcgtgacgaagctccaagcggtttacgccatcaattaaacacaaagtgctg tgccaaaactcctctcgcttcttatttttgtttgttttttgagtgattgggtgggtgat tggttttgggtgggtaagcaggggaaagtgtgaaaaatcccggcaATGGGCCAAGAGGA TCAGGAGCTATTAATTCGCGGAGGCAGCAAACACCCATCTGCCGAGCATCTGAACAATT TGATATTTGAGATCCCCTATCATTGCAGGGTGACAGCGGACGCTTCGCAGAGCTGCATT AACCAGGGCTTCGGGCAGGCCAAAAACTACGGCACGCTCCTGCCACCCAGTCCGCCGGA GGACTCCGGTTCAGGGAGCGGCCAACTAGCCGAGAACCTCACCTATGCCTGGCACAATA TGGACATCTTTGGGGCGGTCAATCAGCCGGGCTCCGGATGGCGGCAGCTGGTCAACCGG ACACGCGGACTATTCTGCAACGAGCGACACATACCGGCGCCCAGGAAACATTTGCTCAA GAACGTTTGCGGCGTGGCCTATCCGGGCGAACTTTTGGCCGTGATGGGCAGTTCCGGTG CCGGAAAGACGACCCTGCTGAATGCCCTTGCCTTTCGATCGCCGCAGGGCATCCAAGTA TCGCCATCCGGGATGCGACTGCTCAATGGCCAACCTGTGGACGCCAAGGAGATGCAGGC CAGGTGCGCCTATGTCCAGCAGGATGACCTCTTTATCGGCTCCCTAACGGCCAGGGAAC ACCTGATTTTCCAAGCCATGGTGCGGATGCCACGACATCTGACCTATCGGCAGCGAGTG GCCCGCGTGGATCAGGTGATCCAGGAGCTTTCGCTCAGCAAATGTCAGCACACGATCAT CGGTGTGCCCGGCAGGGTGAAAGGTCTGTCCGGCGGAGAAAGGAAGCGTCTGGCATTCG CCTCCGAGGCACTAACCGATCCGCCGCTTCTGATCTGCGATGAGCCCACCTCCGGACTG GACTCATTTACCGCCCACAGCGTCGTCCAGGTGCTGAAGAAGCTGTCGCAGAAGGGCAA GACCGTCATCCTGACCATTCATCAGCCGTCTTCCGAGCTGTTTGAGCTCTTTGACAAGA TCCTTCTGATGGCCGAGGGCAGGGTAGCTTTCTTGGGCACTCCCAGCGAAGCCGTCGAC TTCTTTTCCTACGTGGGTGCCCAGTGTCCTACCAACTACAATCCGGCGGACTTTTACGT ACAGGTGTTGGCCGTTGTGCCCGGACGGGAGATCGAGTCCCGTGATCGGATCGCCAAGA TATGGCGACAATTTTGCCATTAGCAAAGTAGCCCGGGATATGGAGCAGTTGTTGGCCAC CAAAAATCTGGAGAAGCCACTGGAGCAGCCGGAGAATGGGTACACCTACAAGGCCACCT GGTTCATGCAGTTCCGGGCGGTCCTGTGGCGATCCTGGCTGTCGGTGCTCAAGGAACCA CTCCTCGTAAAAGTGCGACTTATTCAGACAACGAAGATAAATAAATTTCATTTAACTCG CGAATATTAATGAGATGCGAGTAACATTTTAATTTGCAGATGGTTGCCATCTTGATTGG CCTCATCTTTTTGGGCCAACAACTCACGCAAGTGGGTGTGATGAATATCAACGGAGCCA TCTTCCTCTTCCTGACCAACATGACCTTTCAAAACGTCTTTGCCACGATAAATGTGTTC ACCTCAGAGCTGCCAGTTTTTATGAGGGAGGCCCGAAGTCGACTTTATCGCTGTGACAC ATACTTTCTGGGCAAAACGATTGCCGAATTGCCGCTTTTTCTCACAGTGCCACTGGTCT TCACGGCGATTGCCTATCCGATGATCGGACTGCGGGCCGGAGTGCTGCACTTCTTCAAC TGCCTGGCGCTGGTCACTCTGGTGGCCAATGTGTCAACGTCCTTCGGATATCTAATATC CTGCGCCAGCTCCTCGACCTCGATGGCGCTGTCTGTGGGTCCGCCGGTTATCATACCAT TCCTGCTCTTTGGCGGCTTCTTCTTGAACTCGGGCTCGGTGCCAGTATACCTCAAATGG TTGTCGTACCTCTCATGGTTCCGTTACGCCAACGAGGGTCTGCTGATTAACCAATGGGC GGACGTGGAGCCGGGCGAAATTAGCTGCACATCGTCGAACACCACGTGCCCCAGTTCGG GCAAGGTCATCCTGGAGACGCTTAACTTCTCCGCCGCCGATCTGCCGCTGGACTACGTG GGTCTGGCCATTCTCATCGTGAGCTTCCGGGTGCTCGCATATCTGGCTCTAAGACTTCG GGCCCGACGCAAGGAGTAGCCGACATATATCCGAAATAACTGCTTGTTTTTTTTTTTTA CCATTATTACCATCGTGTTTACTGTTTATTGCCCCCTCAAAAAGCTAATGTAATTATAT TTGTGCCAATAAAAacaagatatgacctatagaatacaagtatttccccttcgaacatc cccacaagtagactttggatttgtcttctaaccaaaagacttacacacctgcatacctt acatcaaaaactcgtttatcgctacataaaacaccgggatatattttttatatacatac ttttcaaatcgcgcgccctcttcataattcacctccaccacaccacgtttcgtagttgc tctttcgctgtctcccacccgctctccgcaacacattcaccttttgttcgacgaccttg gagcgactgtcgttagttccgcgcgattcggttcgctcaaatggttccgagtggttcat ttcgtctcaatagaaattagtaataaatatttgtatgtacaatttatttgctccaatat atttgtatatatttccctcacagctatatttattctaatttaatattatgactttttaa ggtaattttttgtgacctgttcggagtgattagcgttacaatttgaactgaaagtgaca tccagtgtttgttccttgtgtagatgcatctcaaaaaaatggtgggcataatagtgttg tttatatatatcaaaaataacaactataataataagaatacatttaatttagaaaatgc ttggatttcactggaactagtggctgctgctctAAACGACGCATTTCGTAC TCCAAAGTACGAATTTTTTCCCTCAAGCTCTTATTTTCATTAAACAATGAACAGGACCT AACGCACAGTCACGTTATTGTTTACATAAATGATTTTTTTTACTATTCAAACTTACTCT GTTTGTGTACTCCCACTGGTATAGCCTTCTTTTATCTTTTCTGGTTCAGGCTCTATCAC TTTACTAGGTACGGCATCTGCGTTGAGTCGCCTCCTTTTAAATGTCTGACCTTTTGCAG GTGCAGCCTTCCACTGCGAATCATTAAAGTGGGTATCACAAATTTGGGAGTTTTCACCA AGGCTGCACCCAAGGCTCTGCTCCCACAATTTTCTCTTAATAGCACACTTCGGCACGTG AATTAATTTTACTCCAGTCACAGCTTTGCAGCAAAATTTGCAATATTTCATTTTTTTTT ATTCCACGTAAGGGTTAATGTTTTCAAAAAAAAATTCGTCCGCACACAACCTTTCCTCT CAACAAGCAAACGTGCACTGAATTTAAGTGTATACTTCGGTAAGCTTCGGCTTTCGACG
GGACCACCTTATGTTATTTCATCATG ggccagacccacgtagtccagcggcagatcggcggcggagaag ttaagcgtctccaggatgaccttgcccgaactggggcacgtggtgttcgacgatgtgca gctaatttcgcccggctccacgtccgcccattggttaatcagcagaccctcgttggcgt aacggaaccatgagaggtacgacaaccatttgaggtatactggcaccgagcccgagttc aagaagaaggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacg ctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctg gaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcc tttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttc ggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgacc gctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcg ccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctac agagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatct gcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaa caaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaa aaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacg aaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatc cttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtc tgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgtt catccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttacca tctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatc agcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccg cctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaat agtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttgg tatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgt tgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggcc gcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatc cgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgta tgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagc agaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggat cttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcag catcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgca aaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaata ttattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtattt agaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgcgaag
tcttagagccagatatgcgagcacccggaagctcacgatgagaatggccagac

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